TABLE 2.

Interpretation criteria for results of the multiplex HRM methods (assays 1 to 4)

Assay	Mutation site	Tm (°C) value range (95% CIa), base, for SARS-CoV-2 variant strain:
		Delta	Omicron-BA.1	Omicron-BA.2	Omicron-BA.5
1	N	83.1–83.9	83.1–83.9	83.1–83.9	83.1–83.9
	R158G	74.8–75.0, G	74.3–74.4, A	74.3–74.4, A	74.3–74.4, A
	G142D	79.8–79.9, G	79.3–79.4, A	79.3–79.4, A	79.3–79.4, A
2	N	83.1–83.9	83.1–83.9	83.1–83.9	83.1–83.9
	Y505H	79.1–79.2, T	79.6–79.8, G	79.6–79.8, G	79.6–79.8, G
	V213G	74.5–74.6, T	74.5–74.6, T	75.1–75.2, G	74.5–74.6, T
3	N	83.1–83.9	83.1–83.9	83.1–83.9	83.1–83.9
	S413R	73.8–73.9, A	73.8–73.9, A	74.6–74.9, G	74.6–74.9, G
	G446S	78.8–78.9, G	78.1–78.2, T	78.8–78.9, G	78.1–78.2, T
4	N	83.1–83.9	83.1–83.9	83.1–83.9	83.1–83.9
	F486V	74.0–74.2, T	74.0–74.2, T	74.0–74.2, T	74.8–80.0, G
	S704L	78.8–79.0, C	78.8–79.0, C	78.8–79.0, C	78.8–79.0, C

^aCI, confidence interval.