TABLE 3.
LOD of each target calculated using regression probit analysis
| Target | No. of positive results/no. of replicates (%) for each no. of dilution copies/reactions |
LOD,a copies/reaction (95% CIb) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 10,000 | 1,000 | 100 | 50 | 20 | 10 | 5 | 1 | ||
| N | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 9/11 (81.8) | 5/11 (45.5) | 7.38 (4.73–35.16) |
| G142D | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 6/11 (54.5) | 4/11 (36.4) | 9.72 (6.90–22.12) |
| R158G | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 5/11 (45.5) | 3/11 (27.3) | 9.96 (7.31–19.54) |
| Y505H | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 5/11 (45.5) | 3/11 (27.3) | 9.96 (7.31–19.54) |
| V213G | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 5/11 (45.5) | 2/11 (18.2) | 9.50 (7.16–17.1) |
| G446S | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 8/11 (72.7) | 3/11 (27.3) | 7.80 (5.58–16.95) |
| S413R | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 6/11 (54.5) | 3/11 (27.3) | 9.33 (6.80–18.72) |
| F486V | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 10/11 (90.9) | 7/11 (63.6) | 8.25 (4.91–34.98) |
| S704L | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 11/11 (100) | 10/11 (90.9) | 7/11 (63.6) | 8.25 (4.91–34.98) |
a
LOD, limit of detection.
b
CI, confidence interval.