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Chemical structures of N73 microhelixAla variants and their respective aminoacylation activities (12; this study) given in ΔΔG‡ (kcal/mol), which is defined as –RTln[(kcat/KM)variant/(kcat/KM)wild-type]. Aminoacylation assays were carried out with the 2′-deoxynucleotide version of the base analogs M, 2AA and 7DAA. ND indicates that the aminoacylation activity has not yet been determined.
