FIG 3.

Effects of DacA depletion on morphological maintenance and cell growth under alkaline and salt stresses. (A) Increased sensitivity of the dacC mutant to alkaline pH by additional deletion of the dacA gene. The wild-type (WT) and indicated mutant cells were serially diluted from 10⁸ to 10⁴ cells/mL in 10-fold steps and spotted onto an LB plate or LB plates at the indicated pH. (B) Complementation of alkaline sensitivity of the dacC dacA double mutant. The cells of the indicated strains were serially diluted from 10⁸ to 10⁴ cells/mL in 10-fold steps and spotted onto an LB plate and LB plates at the indicated pH. (C) Salt sensitivity of the dacA mutant. The wild-type and indicated mutant cells were serially diluted from 10⁸ to 10⁴ cells/mL in 10-fold steps and spotted onto an LB plate (171 mM NaCl) or an LB plate containing 750 mM NaCl. (D) Morphological defects of the dacA mutant in LB medium. The indicated cells grown in LB medium containing 0.2% arabinose were stained with FM4-64 (red) and then spotted onto a 1% agarose pad. Scale bars, 5 μm.