FIG 4.

Distinct morphological changes of dacC and dacA mutants under alkaline stress conditions. (A) Cell shapes of dacC and dacA mutants under alkaline stress conditions. The indicated cells grown in LB medium or LB media at the indicated pH were stained with FM4-64 (red) and then spotted onto a 1% agarose pad. Scale bars, 5 μm. (B) Complementation of spherical shape of the dacC mutant under alkaline stress conditions. The indicated cells grown in LB medium at pH 9.0 containing 0.2% arabinose were stained with FM4-64 (red) and then spotted onto a 1% agarose pad. Scale bars, 5 μm. (C) Complementation of filamentous shape of the dacA mutant under alkaline stress conditions. The indicated cells grown in LB medium at pH 9.3 were stained with FM4-64 (red) and then spotted onto a 1% agarose pad. Scale bars, 5 μm.