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. 2023 Apr 26;11(3):e00014-23. doi: 10.1128/spectrum.00014-23

FIG 5.

FIG 5

Identified phenotypes of the dacC and dacA mutants are independent of ld-transpeptidases. (A) ld-Transpeptidase-independent alkaline sensitivity of the dacC mutant. The wild-type (WT) and indicated mutant cells were serially diluted from 108 to 104 cells/mL in 10-fold steps and spotted onto an LB plate or LB plates at indicated pH. (B) ld-Transpeptidase-independent salt sensitivity of the dacA mutant. The wild-type and indicated mutant cells were serially diluted from 108 to 104 cells/mL in 10-fold steps and spotted onto an LB plate or an LB plate containing 750 mM NaCl. (C) ld-Transpeptidase-independent morphological changes of the dacC and dacA mutants. The indicated cells grown in LB medium or LB media at the indicated pH were stained with FM4-64 (red) and then spotted on a 1% agarose pad. Scale bars, 5 μm.