FIG 4.

Evaluating decoding center mutants. Abundance scores were determined for plasmid-born 16S rRNA containing separate decoding center mutations A1492U (red), A1493U (blue), and G530C (gray). Scores for mutants were compared to those observed for the parental 16S rRNA (y axis) for the 30S, 70S, and polysome lysate fractions. Error bars represent standard deviations for biological replicates (n = 3). Comparative statistics represent Student's t test results. P values <0.05 (*), < 0.01 (**), < 0.001 (***).