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Growth curve analyses of M. pneumoniae grown in vB10 (serum-free). (A and B) Cell growth in vB10 along with 96 h of culture in 25-cm2 tissue culture flasks containing 5 mL of medium was assessed by (A) protein and (B) DNA biomass quantification and compared to rich medium (Hayflick). Growth performance in vB13 is described in reference 27.
