FIG 6.

Serum-free medium adaptation for M. hyopneumoniae growth. (A) Growth performance of different medium versions obtained during the optimization process of the vB13 formulation for M. hyopneumoniae growth adaptation. Data show the percentage of DNA biomass yield relative to that of rich medium (modified Friis medium) after 96 h in 4-mL cultures. Data represent the mean ± standard error of a minimum of 3 biological replicates except for vB13 (n = 2). Below the bar plot are shown the main contributing factors of medium adaptation. The plus (+) symbol represents the presence of the compound, the minus (−) symbol represents its absence, while +/– indicates a decrease in the concentration of the compound compared to vB13. The detailed formulation of each medium version is shown in Table S5. (B) Growth curve analysis of M. hyopneumoniae in rich medium and serum-free medium vH5 and vH6. Cell growth was monitored over time in 30-mL cultures by DNA biomass quantification. Data represent the mean ± standard error of 3 biological replicates for each medium.