TABLE 1.

Composition of different serum-free medium versions

Component	M. pneumoniae			M. hyopneumoniae
	vB10	vB13	vB13m	vH6
CMRL-1066	0.5×	0.5×		0.5×
l-Cysteine			100 μg/mL
Glucose	7.5 g/L	7.5 g/L	7.5 g/L	1.5 g/L
Glycerol	0.025%	0.025%	0.025%	0.01%
Glutamine	2 mM	2 mM		2 mM
PPLO	15 mg/mL
Yeastolate		10 mg/mL	10 mg/mL	7.5 mg/mL
RNA	1 mg/mL	1 mg/mL
Spermine	10 μg/mL	10 μg/mL		10 μg/mL
Thioctic acid	0.2 μg/mL	0.2 μg/mL		0.2 μg/mL
Pyridoxamine	0.5 μg/mL	0.5 μg/mL
Nicotinic acid	0.5 μg/mL	0.5 μg/mL
Riboflavin	0.5 μg/mL	0.5 μg/mL
Choline	0.5 μg/mL	0.5 μg/mL
Cholesterol	33.3 μg/mL	30 μg/mL	30 μg/mL	30 μg/mL
Phosphatidylcholine	40 μg/mL	40 μg/mL	40 μg/mL	40 μg/mL
Sphingomyelin	40 μg/mL	40 μg/mL	40 μg/mL	10 μg/mL
Palmitic acid	16.6 μg/mL
Oleic acid	20 μg/mL
Nonlipidated BSA	0.33%			0.5%
2-Hydroxypropyl-β-cyclodextrin		5 mg/mL	5 mg/mL
Sodium pyruvate				2.2 g/L
Thymidine				40 μg/mL
Adenosine				10 μg/mL
Cytidine				10 μg/mL
Guanosine				10 μg/mL
Thymine				10 μg/mL
l-α-Phosphatidyl-dl-glycerol				10 μg/mL
1,2-Dipalmitoyl-sn-glycero-3-phosphocholine				20 μg/mL
HEPES	50 mM	50 mM	50 mM
Ampicillin	100 μg/mL	100 μg/mL	100 μg/mL	100 μg/mL
Phenol red (pH 7)	0.0035%	0.0035%	0.0035%	0.0035%
NaOH (to adjust pH to 7.7)