TABLE 2.

Method comparison for BioCode RPP compared with FA-RP results before and after discordant analysis^a

Pathogen	Initial results without discordant analysis						Adjudicated results after discordant analysisb
	TP	TN	FN	FP	PPA (%)	NPA (%)	TP	TN	FN	FP	PPA (%)	NPA (%)
Adenovirus	68	2,528	10	41	87.2	98.4	94	2,534	4	15	95.9	99.4
Bordetella pertussis	2	2,626	0	19	100.0	99.3	16	2,626	0	5	100	99.8
Chlamydia pneumoniae	4	2,642	0	1	100.0	100.0	5	2,642	0	0	100	100
Coronavirus	111	2,492	22	22	83.5	99.1	123	2,506	8	10	93.9	100
Human metapneumovirus	135	2,488	7	17	95.1	99.3	144	2,491	4	8	97.3	99.7
Human rhinovirus/enterovirus	383	2,139	91	34	80.8	98.4	400	2,173	57	17	87.5	99.2
Influenza A	213	2,396	8	25	96.4	99.0	224	2,403	1	14	99.6	99.4
Influenza A H1	0	2,640	0	0	N/A	100.0	0	2,640	0	0	N/A	100
Influenza A H1 pdm09	52	2,578	1	9	98.1	99.7	61	2,578	1	0	98.4	100
Influenza A H3	147	2,474	10	9	93.6	99.6	152	2,481	3	4	98.1	99.8
Influenza B	51	2,579	3	14	94.4	99.5	56	2,581	1	9	98.2	99.7
Mycoplasma pneumoniae	18	2,609	0	20	100.0	99.2	31	2,609	0	7	100	99.7
Parainfluenza virus 1	15	2,630	2	0	88.2	100.0	15	2,632	0	0	100	100
Parainfluenza virus 2	10	2,632	2	3	83.3	99.9	11	2,635	1	0	91.7	100
Parainfluenza virus 3	118	2,508	4	17	96.7	99.3	130	2,509	3	5	97.7	99.8
Parainfluenza virus 4	16	2,627	2	2	88.9	99.9	16	2,629	2	0	88.9	100
Respiratory syncytial virus	200	2,422	4	21	98.0	99.1	204	2,426	0	17	100	99.3

^aResults are no. of specimens unless otherwise indicated. TP, true positive; TN, true negative; FP, false positive; FN, false negative; PPA, positive percent agreement; NPA, negative percent agreement.

^bDiscordant analysis was performed with validated PCR/bidirectional sequencing and/or alternative NAAT and repeat testing with BioCode RPP depending on the availability of residual specimens.