TABLE 1.
Identification of ARV-binding membrane proteins by LC-MS/MSa
| Observed | Mr. |
Delta | Score | Expected value | Match sequence | |
|---|---|---|---|---|---|---|
| Expected | Calculated | |||||
| AnxA2 | ||||||
| 366.2176 | 730.4206 | 730.4225 | −0.0018 | 54 | 0.00041 | K.ELASALK.S |
| 381.2323 | 760.4500 | 760.4517 | −0.0017 | 56 | 0.00014 | K.LMVALAK.G |
| 437.2804 | 872.5461 | 872.5517 | −0.0056 | 69 | 4e-006 | R.KLMVALAK.G |
| 440.7244 | 879.4342 | 879.4338 | 0.0004 | 55 | 0.0003 | R.DLYDAGVK.R |
| 518.7739 | 1,035.5332 | 1,035.5349 | −0.0017 | 61 | 6.4e-005 | R.DLYDAGVKR.K |
| 526.2646 | 1,050.5146 | 1,050.5168 | −0.0022 | 40 | 0.0081 | K.WISIMTER.S |
| 543.7461 | 1,085.4776 | 1,085.4778 | −0.0001 | 62 | 3.1e-005 | K.AYTNFDAER.D |
| 544.3022 | 1,086.5898 | 1,086.5921 | −0.0022 | 74 | 3e-006 | R.DALNIETAIK.T |
| 556.2799 | 1,110.5453 | 1,110.5458 | −0.0005 | 76 | 1.7e-006 | R.QDIAFAYQR.R |
| 611.7999 | 1,221.5852 | 1,221.5877 | −0.0025 | 99 | 1.1e-008 | K.TPAQYDASELK.A |
| 623.3078 | 1,244.6010 | 1,244.5997 | 0.0014 | 102 | 4.7e-009 | R.TNQELQEINR.V |
| 451.8912 | 1,352.6518 | 1,352.6572 | −0.0054 | 63 | 3.9e-005 | K.DIISDTSGDFRK.L |
| 535.5914 | 1,603.7524 | 1,603.7552 | −0.0028 | 69 | 1e-005 | K.SYSPYDMLESIRK.E |
| 545.6219 | 1,633.8440 | 1,633.8423 | 0.0016 | 71 | 5.5e-006 | R.TNQELQEINRVYK.E |
| 547.2712 | 1,638.7917 | 1,638.7923 | −0.0006 | 105 | 2.7e-009 | K.TPAQYDASELKASMK.G |
| 556.9406 | 1,667.8001 | 1,667.8015 | −0.0015 | 59 | 0.00011 | R.SNAQRQDIAFAYQR.R |
| 615.6418 | 1,843.9036 | 1,843.8952 | 0.0084 | 119 | 9.9e-011 | K.LSLEGDHSTPPSAYGSVK.A |
| AnxA5 | ||||||
| 501.3004 | 1,000.5863 | 1,000.5917 | −0.0053 | 61 | 3.9e-005 | K.VLTEIIASR.T |
| 507.7576 | 1,013.5006 | 1,013.5069 | −0.0063 | 32 | 0.045 | R.LYDAYELK.H |
| 400.9010 | 1,199.6813 | 1,199.6873 | −0.0061 | 55 | 0.00014 | R.SEIDLLNIRK.E |
| ADGRL2 | ||||||
| 430.7819 | 859.5492 | 859.5280 | 0.0213 | 26 | 0.029 | K.LQLIAFR.N |
a
ARV, avian reovirus; LC-MS/MS, liquid chromatography-tandem mass spectrometry; AnxA2, annexin A2; ADGRL2, adhesion G protein-coupled receptor Latrophilin-2.