FIG 1.

(A to C) Diagrams of the construction of (A) ChGM-CSF (435-bp), (B) C22-HN (1,716-bp), and (C) ChGM-CSF-HN (2,211-bp). (D to F) Results following construction of the genes (D) ChGM-CSF, (E) C22-HN, and (F) ChGM-CSF-HN in the pAdTrack-CMV backbone and subsequent generation of those genes in the pAdEasy-1 adenoviral backbone plasmid, with a comprehensive unique restriction enzyme digestion analysis carried out in order to determine whether constructs were successful. The T2A peptide from the Thosea asigna virus capsid protein enables the expression of two functional proteins through eukaryotic ribosomes that fail to insert a peptide bond between the glycine and proline residues, yielding separate polypeptides.