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. 2023 May 18;11(3):e00104-23. doi: 10.1128/spectrum.00104-23

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Copyright © 2023 Zhao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — PP2Acα deficiency leads to the presence of polysaccharide granules in Toxoplasma tachyzoites. (A) Transmission electron microscopy of WT, ΔPP2Acα, and ::PP2Acα tachyzoites isolated from HFF-1 cells (top panels) and replicated within HFF-1 cells (bottom panels). Semicrystalline granular deposits are indicated by red arrows (top panel) or red arrowheads (bottom panel). (B) Periodic acid-Schiff staining of WT, ΔPP2Acα, and ::PP2Acα tachyzoites escaped from HFF-1 cells (top panels) and replicated within HFF-1 cells (bottom panels). Tachyzoites or parasitophorous vacuoles are indicated by black arrows and arrowheads, respectively. (C) The activity of PP2A in wild-type (WT), PP2Acα knockout (ΔPP2Acα), and its complemented (::PP2Acα) cells of T. gondii. The data are presented as the mean ± standard deviation (SD) from three independent technical repeats. (D) The numbers of polysaccharide granules observed in WT, ΔPP2Acα, and ::PP2Acα tachyzoites escaped from HFF-1 cells. At least 50 tachyzoites are analyzed in each group. The data are presented as the mean ± standard deviation (SD). (E) Quantification of glycogen in WT, ΔPP2Acα, and ::PP2Acα cells. The data are presented as the mean ± standard deviation (SD). ***, P ≤ 0.001, by a t test.