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. 2023 May 16;11(3):e05121-22. doi: 10.1128/spectrum.05121-22

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Copyright © 2023 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Myeloid cell clusters in the lungs. (A) Immunostaining of CSF1R in the lungs. Scale bars, 20 μm. (B) t-SNE plot of 1,588 mono-macro-neutrophil cells color-coded by their associated clusters. (C) Heatmap of the expression of the top 10 DEGs in each cell cluster. (D) Differences in pathway activities scored per cell by GSVA between myeloid cells isolated from the lungs, with enriched GO terms (P < 0.05). (E) Bar plots showing the proportion of each cluster in vivo in the control, Herts/33, and LaSota groups. (F) Pseudotime trajectory plot representing NDV infection features of the highly virulent NDV Herts/33 strain or the nonvirulent LaSota strain. The solid line indicates features of the nonvirulent LaSota strain, and the dotted line indicates features of the highly virulent Herts/33 strain. (G) Gene expression dynamics model for the nonvirulent LaSota strain and the highly virulent NDV Herts/33 strain. (H) Expression patterns of the top 10 most dynamic genes in two states over pseudotime. (I) Dynamics of the IFN responses in two states over pseudotime.