FIG 4.

Fluorescence monitoring of a mutant strain producing Venus-tagged Sit1 demonstrates production of Sit1 protein during germination under both iron sufficiency and limitation. Aspergillus fumigatus WT and sit1^C-Venus were grown in triplicates under iron limitation (−Fe) and iron sufficiency (+Fe; 0.03 mM FeSO₄) conditions in Aspergillus minimal medium. Individual wells of a 96-well plate filled with 200 μL medium were inoculated with 5 × 10⁶ spores and then incubated to 37°C. Venus-mediated fluorescence was measured hourly over a period of 10 h using a fluorescence microplate reader. The graph shows the mean fluorescence with ± standard deviation (SD) of strain sit1^C-Venus after subtraction of the background fluorescence of WT at the respective time point and of the 0-h time point.