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. 2023 May 24;11(3):e05198-22. doi: 10.1128/spectrum.05198-22

FIG 1.

FIG 1

Standard curve, limit of detection, and electrophoresis results of the polA assay. (A) Standard curve of the polA assay. Plasmids were serially diluted 10-fold to obtain a range of concentrations from 1 × 106 copies/μL to 1 × 100 copies/μL. (B) Limit of detection of the polA assay, with data represented as the mean ± SD of three technical replications. T. pallidum DNA was serially 10-fold diluted to obtain a range of concentrations from 1 × 107 treponemes/mL to 1 × 100 treponemes/mL; 1 × 102 treponemes/mL is equal to 1.93 × 103 copies/mL, 1 × 103 treponemes/mL is equal to 1.42 × 104 copies/mL, 1 × 104 treponemes/mL is equal to 1.19 × 105 copies/mL, 1 × 105 treponemes/mL is equal to 1.60 × 106 copies/mL, 1 × 106 treponemes/mL is equal to 2.04 × 107 copies/mL, and 1 × 107 treponemes/mL is equal to 2.36 × 108 copies/mL. (C) Specificity of the polA assay detected by electrophoresis. The base length of the polA target band is 143 bp. Lane M, 500 bp marker; lane 1, T. pallidum DNA; lane 2, Chlamydia trachomatis DNA; lane 3, Herpes simplex virus DNA; lane 4, Human papillomavirus DNA; lane 5, Neisseria gonorrhoeae DNA; lane 6, human DNA; lane 7, rabbit DNA. Abbreviations: Cp, cross point; R2, coefficient of determination.