FIG 2.

High-throughput screening identified 56 validated hits inhibiting PelA esterase activity in vitro (a). High-throughput biochemical screening data of 68,666 compounds. Each point represents inhibition of PelA esterase activity on the pseudosubstrate, ACC, for a given compound tested at 40 μM. Fluorescent and quenching compounds were omitted. The green dashed line represents the calculated hit threshold (mean + 3σ). (b) Fifty-six validated hits from reconfirmation screen. A validated hit was defined as having ≥25% inhibition in three replicates. Error bars represent the standard error of the mean from three independent trials. ACC, 7-acetoxycoumarin-3-carboxylic acid; σ, standard deviation.