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. 2023 Apr 26;11(3):e00296-23. doi: 10.1128/spectrum.00296-23

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Copyright © 2023 Razvi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — SK-17154-O is a noncompetitive inhibitor. (a) Reversibility test using gel filtration testing SK-017154-O. Assay mixtures contained 1 μM PelA, 25 μM compound, and 400 μM AMMU. Error bars represent the standard error of the mean from six independent trials. Statistical significance was evaluated using an ordinary one-way analysis of variance with Tukey corrections for multiple comparisons. (b) Inhibition kinetics by SK-017154-O generally fit to the Michaelis-Menten equation. Assay mixtures contained 1 μM PelA. (c and d) Apparent V_max (c) and apparent K_m (d) as a function of SK-017154-O concentration; (e) data in panel a fit to a global noncompetitive inhibition model. Error bars represent the standard deviation from two independent trials. (f) Kinetic scheme of noncompetitive inhibition. RFU, residual fluorescence units; AMMU, acetoxymethyl-4-umbelliferone.