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. 2000 Jul 1;28(13):2503–2511. doi: 10.1093/nar/28.13.2503

Figure 2.

Figure 2

Figure 2

TSH effect on Hex mRNA expression. (a) FRTL-5 cells were kept in the presence or absence of TSH (1 mU/ml) for 5 days. Hex, Pax8 and GAPDH mRNAs were detected by northern blotting. Filters were exposed to a phosphorimager for 24 h. Signals were quantitated using the Multi-Analyst computer program and normalized to the corresponding GAPDH values. The Hex:GAPDH ratio in the presence of TSH was considered arbitrarily as 100. Each bar represents the mean value ± SD of three independent experiments. (b) Representative autoradiograms.