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. 2000 Jul 1;28(13):2503–2511. doi: 10.1093/nar/28.13.2503

Figure 6.

Figure 6

Figure 6

Hex binding to sequences of the Tg promoter. (a) Oligonucleotides used in the gel retardation assay. The 5′-TAAT-3′ and 5′-CAAG-3′ sites are boxed. The underlined bases in the A oligonucleotide correspond to 5′-CAAG-3′ in the bottom strand (not shown). The black dots below the Cβ oligonucleotide indicate the two bases which differ between the C and Cβ oligonucleotides. (b) Binding of Hex, Antp and TTF-1 HDs to the A, C, BS2 and Cβ oligonucleotides. The gel retardation assay was performed as described in Materials and Methods. B, protein-bound DNA; F, free DNA.