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. 2023 Jun 9;10(6):ENEURO.0366-22.2023. doi: 10.1523/ENEURO.0366-22.2023

Figure 2.

Figure 2.

Development of dendritic spines in dSPNs and iSPNs of the dorsomedial striatum. A, Schematic drawings show the regions of the dorsomedial striatum that are included for analysis. B, C, Immunohistochemistry of eYFP shows the development of eYFP-labeled dendritic spines in dSPNs and iSPNs, respectively, in the dorsomedial striatum (caudoputamen) of Drd1-Cre mice (B) and Adora2a-Cre mice (C). D, Quantification of spine density. E, Quantitative analysis of morphologic profiles of spines during development. F, Dynamic changes of specific types of spines during development. * differences between ages. *p < 0.05, **p < 0.01, ***p < 0.001. # differences between genotypes. ##p < 0.01. Two-way ANOVA is used in D. One-way ANOVA followed by Tukey’s HSD post hoc tests is used in F for the data that are normally distributed. Kruskal–Wallis one-way ANOVA followed by Dunn’s pairwise multiple comparisons tests are used in F for data that are not normally distributed. Data in D and E are mean ± SEM; Stubby and Thin/Filopodial spine data in F are mean ± SEM; Mushroom, Branched, Multi-branched and Atypical spine data in F are median ± interquatile range. n = 10 cells from 2–3 mice/age. P, postnatal day. Scale bar: 10 μm.