Skip to main content
. 2023 Jun 14;7(7):e00174. doi: 10.1097/HC9.0000000000000174

FIGURE 2.

FIGURE 2

Western diet plus single ethanol binge synergistically increases hepatic steatosis. The same mice described under Figure 1 were used. (A) Serum levels of NEFA, TG, and total cholesterol (TC) at necropsy. (B–E) Oil red O (B and C) and LipidSpot (D and E) staining and quantification of hepatic lipid droplets. (F) Hepatic contents of TG, total cholesterol (TC), free cholesterol (FC), and phospholipids (PL). (G) Hepatic levels of mRNAs for key bile acid synthetic enzymes. (H) Serum concentrations of total bile acids (BA). (I) Hepatic contents of total bile acids (BA). *p < 0.05 (Western diet or HFD vs. chow diet); # p < 0.05 (binge ethanol vs. maltose solution); & p < 0.05 (HFD vs. Western diet). Abbreviations: C, Chow diet; W, Western diet; H, High-fat diet. B, Ethanol binge. eWAT, epididymal white adipose tissue; iWAT, inguinal subcutaneous white adipose tissue; iBAT, interscapular brown adipose tissue.