Fig 1. Circulation of yellow fever virus in Minas Gerais, 2016 to 2021.

A: Map of Minas Gerais showing the location of Curvelo, where yellow fever virus was detected in a Callithrix sp specimen, in 2020 (purple), municipalities that had cases of yellow fever affecting human and or non-human primate, 2016–2018 (green), and municipalities that had yellow fever epizootics confirmed from Jul 2020 to Jun 2022 (grey). The inset map displays the location of Minas Gerais in Southern Brazil. B: Phylogenetic tree reconstructed with using partial envelope sequence of yellow fever virus (1000nt). The sequence of yellow fever virus detected in a Callithrix sp specimen (NHP1041), in 2020, from Curvelo is shown in purple. Some branches were collapsed for clarity. The phylogenetic tree was reconstructed using the maximum likelihood method, nucleotide substitution model TN93 +I, with 1000 bootstrap replicates, using PhyML. The final tree was edited and visualized using iTOL. The map was generated using shapefiles “MG_Municipios_2020.shp” and “BR_UF_2020.shp” (https://www.ibge.gov.br/geociencias/organizacao-do-territorio/malhas-territoriais/15774-malhas.html?=&t=acesso-ao-produto) and QGIS, a free open source (GNU General Public License) system developed by Open Source Geospatial Foundation (https://www.qgis.org/pt_BR/site/forusers/download.html).