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a–e) Michaelis-Menten plots of AvPAL* and high active variants combined with N453S. L4P-G218S-N453S and T102R-M222L-D306G-N453S did not exhibit any activity at any of the Phe concentrations tested. Kinetic parameters are listed in Table S3. f) Whole cell conversion assay indicates that active enzymes are more active when encapsulated in E. coli cells even though there do not display superior kinetic parameters.
