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. 2000 Jul 1;28(13):2467–2472. doi: 10.1093/nar/28.13.2467

Figure 4.

Figure 4

A model for integration of the I factor generating a tandem repeat at the 3′ end of the element (see text). (A) The I factor encoded endonuclease cleaves chromosomal DNA at the target for integration (arrows). (B) The RNA transposition intermediate anneals to the target DNA. (C) Reverse transcription initiates using the chromosomal DNA as primer and RNA as template. (D) The RNA template dissociates from the chromosomal DNA and anneals to the newly synthesized sequence. (E) Reverse transcription extends into the I factor to complete first DNA strand synthesis. (F) After degradation of the RNA by RNase H, second strand synthesis is completed to generate a tandem duplication and the target site duplication. The target DNA is indicated in black, the RNA transposition intermediate in red, the first cDNA strand in blue and the second cDNA strand in green. The two copies of the tandem repeat generated at the 3′ end of the I factor are shown in italic and bold. The target site duplication is underlined.