Fig. 2.

Discriminatory power of plasma miRNAs for urinary albumin excretion. (a) Plasma levels of miR-374a-5p, miR-1260b and miR-126–3p in testing cohort 1025 participants, 83 with urinary albumin excretion (UAE). The microRNA (miRNA) levels have been quantified by real-time quantitative polymerase chain reaction (RT-qPCR), expressed as log10-transformed of absolute copy number and was normalized to miR-125a and miR-186 as internal controls. The miR-374a-5p, miR-1260b and miR-126–3p were significantly increased in UAE subjects. Data was compared using the Mann-Whitney U test. The ends of the boxes indicate the quartile 1(upper) and 3 (down), the middle line represents the median, and the whiskers are the minimum and maximum values of the data series (b) The areas under the curve (AUC) and receiver operating characteristics (ROC) curves were calculated for plasma miRNAs in the testing cohort. The diagnostic signature was calculated by a logistic regression model. The AUC of 3-miRNAs and 2-miRNAs signatures were significantly higher when compared to individual miRNAs (p < 0.05). (c) ROC curves are shown for individual and miRNA signatures.