Figure 2.

The N-terminal region of ZF, rich in negatively charged amino acids, is an activation domain. On the left is the schematic representation of the structure of the ZF protein. The numbers indicate the positions of the amino acid. ZF and its deletion mutants were fused to the GAL4 DNA-binding domain. The same amount (0.5 µg) of each plasmid was introduced into COS7 cells along with the reporter plasmid pG5EC (0.5 µg), which has five copies of the GAL4 UAS in the promoter region linked to the CAT gene. The parental vector expressing only the GAL4 DBD, pM1, was used as the blank control. The CAT activity was measured by ELISA 48 h post-transfection.