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. 2000 Jun 15;28(12):2446–2454. doi: 10.1093/nar/28.12.2446

Figure 6.

Figure 6

ZF prevents HSV ICP0 expression in virus-infected cells in a HCF-dependent manner. Vero cells were transfected with plasmids expressing either ZF (A–C) or ZF Y224A (D–F). The cells were then infected with HSV-1 at a m.o.i. of 10 p.f.u./cell. Six hours after infection the cells were fixed and stained simultaneously for both ZF (Alexa546, red) and ICP0 (Alexa488, green). The cells were then visualized in a fluorescent microscope using either a 546 (A and D) or 450–490 nm filter (B and E). The red and green images were combined (C and F) using Northern Eclipse 5.0 software. In (E) the arrowheads indicate ICP0 stained nuclei that also stain for ZF Y224A.