Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 15;13:9737. doi: 10.1038/s41598-023-36654-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Molecular analysis of the FGF14 repeat locus. (A) Fluorescent long-range PCR of the FGF14 repeat locus of four patients with late-onset cerebellar ataxia. The calculated number of repeat units of each allele (before correction) is indicated for each of the four patients. (B) 5′ RP-PCR and 3′ RP-PCR of the FGF14 repeat locus of two patients carrying a (GAA)_≥250 repeat expansion in FGF14. (C) Sanger sequencing of a patient carrying a (GAA)_≥250 repeat expansion showing GAA repeats: (GAA)_n on forward strand and (TTC)_n on reverse strand. (D) Agarose gel electrophoresis (1.5%), lanes 1 and 8: 2,000 bp molecular weight marker, 2: 17/132 repeat units, 3: 9/284 repeat units, 4: 9/510 repeat units, 5: 9/313 repeat units, 6: 9/467 repeat units, and 7: negative control. (E) Agarose gel electrophoresis (1.5%), lanes 1 and 9: 2,000 bp molecular weight marker, 2: 107/236 repeat units, 3: 40/132 repeat units, 4: 16/182 repeat units, 5: 8/9 repeat units, 6: 17/319 repeat units, 7: 43/370 repeat units, and 8: 28/94 repeat units. Agarose gels were imaged with the Uvidoc HD6 Gel Documentation System using the default UV-Gel settings in the Uvitec-1D software, which resulted in overexposure. The gels presented in D and E were cropped for clarity of presentation. The original gels are presented in Supplementary Fig. S10.