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. 2023 Jun 15;14:3561. doi: 10.1038/s41467-023-39085-1

Fig. 1. Overview of automated AFM acquisition system.

Fig. 1

a Technical drawing of motor mount for interfacing servo motors with AFM translation knobs. b Assembled motor mounts. The Stage Frame slides along the edge of the stage (orange arrows) while the Motor Frame slides along the Alignment Rods, towards and away from the Stage Frame as the knobs turn (blue arrows). c Schematic of AutoAFM feedback system. d Example of AutoAFM feedback with desired AFM sampling positions (blue), actual AFM positions (red), and AFM path of movement and positions outside of the desired positions (orange). Representative images of AutoAFM collecting AFM measurements over a whole tissue (e) and a region of interest (f) in a breast tumor section. 200 experiments were conducted obtaining reproducible results. Scale bar, 100 μm.