Figure 5: Protein Array Analysis of a In Vivo Xenograft Tumor Model of EGFR TKI Resistance.
Mice were inoculated with H1650 human lung cancer cells. Mice began treatment when tumors became palpable (2–3mm diameter) and were treated every day with vehicle control or 50mg/kg lapatinib. Protein was collected from H1650 tumors (N=5), samples were pooled equally, and the PhosphoExplorer antibody array was performed (Full Moon Biosystems). RFU for all significantly differently expressed proteins is shown (N=2). A 2-Way ANOVA and Bonferroni post hoc tests were used to determine significance. * = p<0.05, ** = p<0.01, *** = P<0.001.