Figure 6:

Schematic description of the Agena iPLEX SMA assay design consisting of a target specific PCR reaction, then a single base extension with molecular weight modified dideoxynucleotide terminators of the extension primer, annealing directly upstream of the subject polymorphic site. Here, the two well defined positions c.840 and c.1155 of SMN1 and SMN2 genes were utilized as targets for PCR and single base extension primer design. Black arrows represent multiplex PCR while blue arrows represent multiplex extension primers (reprinted with permission from Lin et al., 2019, copyright 2019 Frontiers Media S.A.).