. Author manuscript; available in PMC: 2024 Jul 1.

Published in final edited form as: Mass Spectrom Rev. 2022 Dec 16;43(4):683–712. doi: 10.1002/mas.21826

Table 1:

Summary of MS/MS acquisition precursor/product ions for Hb peptides and internal standards used for SCD NBS protocol for identification of clinically relevant beta-chain hemoglobinopathies (reprinted with permission from Moat et al., 2017, copyright 2017 SAGE Publications).

Target peptide	Tryptic peptide and ion	Parent/daughter ions (m/z ratios)	Hb variant peptide ratios
HbS	βT1 y4	461.66/472.15	SβT1 y4/wt βT1y4 (S:A)
HbC	βT1 b4	694.30/451.15	CβT1 b4/wt βT1y4 (C:A)
HbD^Punjab	βT13 b3	689.22/377.08	DβT13 b3/wt βT13 b3 (D^Punjab:A)
HbO^Arab	βT13 y9	625.25/1001.45	OβT13 y9/wt βT13 y9 (O^Arab:A)
HbE	βT3 y6	458.65/604.15	EβT3 y6/wt βT3 y9 (E:A)
HbLepore	δT2y6	480.16/688.20	δT2 y6/wt βT2 y6 (Lepore:A)
HbF	γT2 y6	488.3/691.30
HbA	wt βT1 y4	476.67/502.15	wt βT1 y4/HbFγT2 y6 (A(βT1):F)
	wt βT2 y6	466.76/675.38	HbFγT2 y6/wt βT2y6 (F:A (βT2))
	wt βT3 y9	657.70/887.25	wt βT3 y9/HbFγT2 y6 (A(βT3):F)
	wt βT13 b3	689.72/378.05
	wt βT13 y9	689.85/1001.35
Internal Standard		465.65/480.15