cDNA sequence encoding E.gracilis Cbf5p, displayed with the deduced amino acid sequence. Locations of the trans-spliced leader sequence, TruB motifs I and II, and PUA domain (36,84) and the C-terminal KKE repeat are indicated. Alternative polyadenylation sites determined by 3′ RACE (see Fig. 1) are denoted by asterisks. Because in the gene sequence A residues immediately follow one of the deduced polyadenylation sites, the position of this site was assigned tentatively.