Table 1. Oligonucleotides used for cloning of Cbf5p cDNA and genomic sequences.
| Primer | Sequence (5′ to 3′) | Corresponding peptide sequence |
|---|---|---|
| P-4 | 5′-AATAAAGCGGCCGCGGATCCAA-3′ | |
| dTP-4 | 5′-AATAAAGCGGCCGCGGATCCAAT16-3′ | |
| P-16 | 5′-AATAAAGCGGCCGCGGATCCAAT16(A/G/C)N-3′ | |
| P-22 | 5′-CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGT-3′ | |
| P-23ddC | 5′-pACCTGCCddC-3′a | |
| P-24 | 5′-GGATCCTAATACGACTCACTATAGGGC-3′ | |
| P-25 | 5′-AATAGGGCTCGAGCGGC-3′ | |
| P-55 | 5′-GGAGCTCAATAAAGCGGCCGC-3′ | |
| P-70 | 5′-AGTCA(T/C)GA(A/G)GTN(T/G)CNTGG-3′ | SHEVV(A/S)W (sense) |
| P-73 | 5′-ACTTTTGGN(A/T)C(C/A/G)A(A/G)NGTNCC-3′ | GTL(D/V)PKV (antisense) |
| P-77 | 5′-GCT(A/G)T(T/C/G)TG(T/C)TA(T/C)GGNGCNAA-3′ | A(I/V)CYGAK (sense) |
| P-79 | 5′-ATNGC(C/T)TCNCC(T/C)TTNGTNGT-3′ | TTKGEA(I/V) (antisense) |
| P-85 | 5′-TTGAAGCGAATCCTGAAGGTGGAGAAGAC-3′ | |
| P-87 | 5′-GATGACCTCGTTGACCTCAATGCCGT-3′ | |
| P-86 | 5′-AAGGTGGAGAAGACTGGGCACGCA-3′ | |
| P-88 | 5′-CAATGCCGTTCTCAAAGCGGAGGAGT-3′ | |
| P-95 | 5′-CCAATCGGGTGGCTCTCTCAATGCAGA-3′ | |
| P-99 | 5′-CTCGGCATCGCCCTCATGACCA-3′ | |
| P-107 | 5′-CTGCGGCAGCAACGAGGAGGCGATGAA-3′ | |
| P-163 | 5′-GGCATAGCTTCAGGCCGTAGGTCATTCA-3′b | |
| P-164 | 5′-CCCCTGGAACGTGCGGTGCTGCTGCTT-3′c |
a3′ end blocked with enzymatically added dideoxy C (ddC), rather than an amine group as in ref. 56.
bCorresponds to positions 1469 to 1496 of the cDNA sequence.
cCorresponds to positions 1511 to 1537 of the cDNA sequence.