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Diagram showing reagents plating format for the C18 stage tip clean up in a Lobind 96 well plate. This step is manual and not performed by KingFisher Duo. Conditioning buffer: 50%ACN/0.1% TFA. Wash buffer: 0.1% TFA. HLA-I Elution Buffer: 28% ACN/ 0.1% TFA. HLA-II Elution Buffer: 32% ACN/ 0.1% TFA.
