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. 2023 Jun 2;17:1166480. doi: 10.3389/fncel.2023.1166480

FIGURE 4.

FIGURE 4

Comparison of GRABNE fluorescence and electrophysiological responses to two consecutive norepinephrine application. (A) Fluorescence responses of GRABNE-expressing cells to a brief drug puff (10 ms) application of 200 μM NE, 10 mM ACh, 100 μM 5-HT and ACSF, respectively. (B) Electrophysiological responses of CA1, CA3, and DG neurons to a brief (10 ms) puff application of 200 μM NE. (C) Fluorescence responses of control non-expressing (left) and GRABNE-expressing (right) CA1 neurons to a brief puff (10 ms) application of ACSF and 200 μM NE. (D) Fluorescence (upper panel) and electrophysiological (lower panel) responses of non-expressing (left) and GRABNE-expressing (right) CA1 neurons to two consecutive puff (10 ms) of 200 μM NE. (E) Values for the two consecutive fluorescence responses of non-expressing (first: 0.13 ± 0.02%; second: 0.11 ± 0.01%; p = 0.10; n = 9) and GRABNE-expressing (first: 10.45 ± 2.75%; second: 10.41 ± 2.43%; p = 0.65; n = 9) CA1 neurons. (F) Values for the two consecutive adrenergic currents in non-expressing (first: 23.80 ± 2.95 pA; second: 13.63 ± 1.48 pA; p = 0.0039; n = 9) and GRABNE-expressing (first: 25.10 ± 3.21 pA; second: 13.75 ± 1.59 pA; p = 0.0078; n = 9) CA1 neurons. Data are shown as mean ± SEM. **p < 0.01, two-tailed Student’s paired t-test.