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. 2023 Jun 2;14:1200195. doi: 10.3389/fimmu.2023.1200195

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Copyright © 2023 Sarker, Dhama and Gupta

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multiple sequence alignment of non-structural (NS) proteins of DENV serotypes 1 to 4. The amino acid sequences of (A) NS1 (B) NS2A and NS2B (C) NS3 (D) NS4A, 2K and NS4B (E) NS5 are taken from polyprotein having accession no. QTH36735.1, NP_056776.2, YP_001621843.1, NP_073286.1 for DENV serotypes 1 to 4 respectively. Fully conserved amino acids are indicated with black dots while variable amino acids are displayed in the Clustal Omega format using Jal view. As per the Global Similarity (BLOSUM62) analysis, the sequence similarity index for NS1, NS2, NS3, NS4 and NS5 proteins are 0.77 ± 0.03, 0.51 ± 0.04, 0.82 ± 0.03, 0.75 ± 0.02, and 0.79 ± 0.02, respectively.