Table 3.

shows the six components necessary to conduct RT-qPCR for human enterovirus quantification. The final concentration and volume of the reagent; master mix, probe, reverse primer (RWD), forward primer (FWD), genetic material (RNA) and distilled water (ddH₂O) are presented. Altogether the total volume for each reaction is 15 µl.

Reagents	Concentration	Amount (µl)
Master mix	1X	7.5
ddH2O	1X	2.6
Probe	300 nM	1.8
RWD primer	400 nM	1.2
RNA	1X	1.0
FWD primer	300 nM	0.9