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. 2023 Jun 2;11:1152510. doi: 10.3389/fcell.2023.1152510

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Copyright © 2023 Vieira, Raymond, Kelley, Cherubino, Sahin and McCusker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterizing anterior/posterior positional information in the bulbous mass using the ALM assay. (A) Cartoon representing the surgical procedure where bulbous mass tissue form a GFP + animal (green) is grafted to innervated anterior or posterior limb wound sites. Cartoons below explain the possible phenotypes in this assay and how they are interpreted. (B) Live images of the bulbous masses where grafts were obtained form (red dotted line outlines the location where the graft was taken) at 3 and 21 weeks (W3, W21) post-surgery. Host locations are indicated; where “P host” is a posterior wound site, and “A host” is an anterior wound site. (Right) Images of whole mount skeletal preparations of ectopic limb structures (red arrows). Full phenotype data is provided in Table 3.