TABLE 1.

qRTPCR primer sequences.

Gene	Forward primer (5′to 3′)	Reverse primer (5′to 3′)
Alx4	GGA​CAC​CCA​GAA​TAC​TGA​GCA	TTA​AGT​GCC​CTG​TCA​TGT​GG
BMP2	ATG​CCA​TCG​TGC​AGA​CTT​TG	ACC​ACC​TTT​TCG​TTC​TCG​TC
Chrdl1	GAG​CCC​TAC​GGA​TTG​GTG​TA	GTG​GAT​AGG​GTT​GGC​ACA​GT
Ef1-a	AAC​ATC​GTG​GTC​ATC​GGC​CAT	GGA​GGT​GCC​AGT​GAT​CAT​GTT
FGF8	AGCTGATTGGCAAGAGCA	GTG​AAG​GCC​ATG​TAC​CAT​CC
Gli3	CAT​GGA​TGT​GGT​CGT​TAT​TGA​TGT​G	GAG​GTT​ATT​TAC​GAG​ACC​GAC​TGT​C
Grem1	GGA​CAC​CCA​GAA​TAC​TGA​GCA	GTA​GAC​CAA​TCG​AAA​CAT​CCT​GT
Hand2	CCA​GCT​ACA​TCG​CCT​ACC​TC	AGC​TCT​TTC​TTG​CGC​TTG​TC
HoxA9	CTG​GGC​CTT​TTA​TTG​TGC​AT	GTG​ACA​TCT​GAG​TGC​GTT​CG
HoxA11	GTG​TTG​CCA​AAC​AGG​CTT​GG	TAG​TCA​TTT​CCT​GCA​CGA​AGG​TC
HoxA13	CAG​GAG​GGT​CAA​AGA​GAA​GAA​GG	TCT​GGT​GAC​CAA​TGA​ACA​GCT​TC
HoxC5	CGG​CAG​ATC​AAA​ATC​TGG​TT	GCC​AGA​ATG​ACC​GAT​TCA​CT
Ptch1	TGT​AGA​TCT​GCT​CCA​ATG​CAA​AC	CTG​ACC​CGG​AGT​ACT​TGC​AG
Shh	AGT​GAC​CAG​ACC​GGT​GAA​AG	CGC​TCC​GTC​TCT​ATC​ACG​TA
Tbx5	CTG​GAA​GGC​GCA​TGT​TTC​CAA​GTT	TGG​CGA​ATC​CGG​ATG​GAC​GTA​TAA