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. 2023 Jun 2;14:1139391. doi: 10.3389/fimmu.2023.1139391

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Copyright © 2023 Ding, Sheriff, Sontz and Merchant

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Precipitated SLFN4 complex exhibits GTPase activity. (A) Immunoprecipitation of SLFN4. Extracts from RAW 264.7 cells treated with IFNα (800U/ml) for 16h were immunoprecipitated using an anti-SLFN4 antibody followed by immunoblotting using anti-SLFN4 to confirm the presence of SLFN4 in the cellular lysate. (B) GTPase-Glo assay (Promega) shows that the precipitated SLFN4 complex exhibits GTP hydrolyzing activity in a dose dependent manner. N=4 experiments.