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. 2023 Jun 2;14:1139391. doi: 10.3389/fimmu.2023.1139391

Figure 8.

Figure 8

Genetic deletion of Slfn4 in Gli1+ cells or Sildenafil treatment mitigated Helicobacter-induced metaplasia and reversed T cell suppression. Representative images of gastric mucosa from uninfected WT mice (UI), 4-month-infected WT mice (Hf), Gli+CreERT2+/Slfn4fl/fl mice and mice with sildenafil gavage by (A) H&E staining and (B) IF staining for ATP4b (white), GSII (green), gastric intrinsic factor (red). Scale bar, 100 μm. Corpus metaplasia weighted score for N= 5 mice per group. One-way analysis of variance (ANOVA) followed by Friedman test was performed. (C) Representative western blot images of SLFN4 and NOS2 proteins with TUBULIN used as a loading control. qPCR of (D) stomach Slfn4 and NOS2. (E) flow analysis of stomach immune cells on T cell marker CD4 and CD8. (F) TNFα and IL1α cytokine levels in the stomachs were measured by ELISA. Kruskal-Wallis one-way ANOVA with Dunn’s test for multiple comparisons. Horizontal lines represent the median and interquartile range for n = 5 mice per group. *P <0.05 compared to Hf infected group. **P<0.01.