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. 2023 Jun 2;17:1130169. doi: 10.3389/fnana.2023.1130169

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Copyright © 2023 Wang, Yuan and Taché.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Capillary rings and nerve fibers at the top of the mucosa. The samples were from the mid-colon. The vessels were painted by WGA perfusion (cyan), nerve fibers were immunolabeled by PGP9.5, CGRP, and VIP (red), and the tissues were counterstained by DAPI (blue). The different fluorescent channels merged in the same image demonstrate the mucosa capillary net in relation to immunofluorescent nerve terminals. High magnifications (objective 63X) of double labeling of WGA with each immunostaining are in the bottom panels.