Figure 7.

A photomicrograph of triple fluorescent labeling of vessels and nerves in the submucosa of a mouse mid-colon shown in different panels with various fluorescent labeling. The vessels were painted by WGA perfusion (cyan) combined with CD31 immunolabeling (red), and nerves by PGP9.5 immunostaining (purple). The arteries (a), veins (v), and lymphatic vessels (lym) accompanied each other. WGA perfusion did not stain the veins. Nerve fibers traveled along the arteries. The lower-left panel is enlarged in the area framed in the panel earlier, demonstrating the nerve fibers traveling along the blood vessels. The single fine nerve fibers adjacent to the vascular wall (arrows) were possibly those innervating the vessels.