Fig. 3.

Box-and-violin plots showing the relationship between small plasmid recovery and (a) average long-read depth of coverage, (b) average long-read read quality, (c) plasmid size, and (d) the ratio between the average long-read length and the length of the associated plasmid. ‘Frequently absent’ plasmids include those not recovered in at least one third of all long-read-only replicate assemblies, whereas ‘frequently present’ plasmids were recovered in at least one third of the replicate assemblies. Significant differences were determined using a two-sided t-test (α=0.05).