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. 2023 May 25;9(5):mgen001016. doi: 10.1099/mgen.0.001016

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Fig. 6. — (a) Minimum spanning tree of 113 pOXA-48-like plasmid sequences demonstrates high nucleotide conservation, as well as some divergent sequences. Plasmid sequences included in the tree, which were obtained by mapping short reads to pOXA48a (GenBank accession no. JN626286) [35], are from isolates with ≥70 % mapping to this reference sequence. The tree was reconstructed from a SNP distance matrix. The branch lengths are displayed using a log scale and indicated on the branches. The nodes are partitioned by (and their sizes proportional to) the number of represented isolates. Isolates are coloured by ST ( K. pneumoniae species complex only) with the number of isolates from each ST indicated in square brackets. Isolates marked with a black filled circle were selected for long-read sequencing to enable complete plasmid sequencing. (b) The same tree as in (a) coloured by species of the host isolate or replicon type identified from the genome. (c) The major node from the tree in (a) coloured by ST, with annotations marking the sampling hospitals.