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. 2023 May 8;27(12):1682–1696. doi: 10.1111/jcmm.17765

FIGURE 2.

FIGURE 2

XIAP inhibition impairs cell viability and clonogenic capacity in AML cells. (A) After treatment with various concentration of birinapant for 0, 24, 48, 72 and 96 h, the cell viability of two AML cell lines HEL and THP‐1 as well as patient blast cells was measured by CCK‐8 assay. (B) After transfection with si‐Control or si‐XIAP‐3 for 48 h, the cell viability of HEL and THP‐1 cells as well as patient blast cells was determined by CCK‐8 assay. (C) The colonies were observed under a microscopy in HEL and THP‐1 cells as well as patients blast cells after treatment with or without birinapant at 20 μM for 8 days or 14 days. (D) The colonies were observed in HEL and THP‐1 cells after transfection with si‐Control or si‐XIAP‐3 for 8 days. Representative images were shown, and scale bar represents 50 μm. Results are expressed as mean ± SD representing at least three independent experiments. Ns and ** represent p > 0.05 and p < 0.01, respectively.