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. 2023 Mar 2;207(12):1565–1575. doi: 10.1164/rccm.202209-1707OC

Figure 4.

Figure 4.

IL-18, IL-33, and airway epithelial cells (AECs) induce IFN-γ and type-2 (T2) gene expression in eosinophils (EOS). (A–C) Quantitative PCR analysis for expression of IFNG (A), IL13 (B), and IL18 (C) (in comparison with the housekeeping gene HPRT1) in EOS isolated from the peripheral blood of four human donors cultured for 2 hours in the presence or absence of IL-18 (10 ng/ml) or IL-33 (10 ng/ml). Individual data points represent the mean value of four EOS replicates per EOS donor; each replicate was the result of three PCR reactions. Mean values are indicated, with error bars representing the SEM. (D and E) Quantitative PCR analysis for expression of IFNG (D) and IL13 (E) in EOS isolated from the peripheral blood of three human donors cultured for 8 hours in the presence or absence of primary AECs from a single healthy adult donor. Individual data points represent the mean value of three EOS replicates per EOS donor; each replicate was the result of three PCR reactions. Mean values are indicated, with error bars representing the SEM. P values represent the result of two-way ANOVAs. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.